陈时盛 小麦抗病遗传育种实验室主任
邮箱：shisheng.chen@pku-iaas.edu.cn / shi-shengchen@hotmail.com
研究方向：小麦及其近缘植物优异基因的发掘与利用、关键基因的克隆及功能研究、小麦种质创新及新品种选育
个人简介：
2009.09 - 2015.06，四川农业大学，Ph.D.（博士学位）
2012.11 - 2014.10，美国加州大学戴维斯分校（University of California, Davis），国家公派联合培养博士
2015.01 - 2019.03，美国加州大学戴维斯分校（University of California, Davis），博士后
2019.04 – 至今，北京大学现代农业研究院，研究员，课题组长（PI）
主要荣誉：
2023年 山东省先进工作者
2022年 国家级重点人才工程
2022年 山东省“泰山学者”青年专家
2020年 潍坊市“鸢都产业领军人才”
研究工作与成果：
本实验室以小麦为研究对象，利用正向与反向遗传学、转录组学、基因组学、分子和生化等方法解析小麦重要功能基因，特别是小麦真菌病害抗性基因。开展基因的定位、克隆和功能分析，并进行小麦的遗传改良和种质创新。重点包括：1、小麦锈病抗性基因的挖掘与定位、关键抗病基因的克隆、抗病分子机制的研究；2、小麦产量、发育相关基因克隆，功能机制的研究；3、小麦种质资源评价和小麦种质创新。
课题组已成功从小麦及其近缘种属克隆到了7个抗锈病新基因，包括来自于拟斯贝尔托山羊草的Lr47，一粒小麦T. monococcum的Sr21、Sr60和Sr22b；来源于四倍体小麦的Sr13、Sr9和Yr15（参与）。解析了4个穗部或株型发育相关基因HB2、TaAPA2、BGI1和WAPO-A1（参与）。此外，先后发掘定位了Sr60、SrTm4、SrTm5、SrKN、LrKP等10个新型抗病基因，正式命名了Sr60、Sr22b等基因。在Nature Communications、PNAS、New Phytologist、Plant Biotechnology Journal等国际主流刊物上发表了SCI论文30多篇，获批国家发明专利7项；主持国家、省、市科研项目10余项，申请植物新品种权4项。
课题组常年招聘科研助理、副/助理研究员，欢迎加盟！
代表性论文：
(第一作者*；通讯作者#)
32. BaiS*; WangG*; SongR; …ZhaoL; XueY; WangZ#; ChenS#. Mutations in wheat TaAPA2 gene result in pleiotropic effects on plant architecture. SCIENCE CHINA-LIFE SCIENCES2024,67:2039–2042
31. XuB*; ShenT*; ChenH*; LiH; …ChenG; HaoM#; ChenS#. Mapping and characterization of rust resistance genes Lr53 and Yr35introgressed from Aegilops species. Theoretical and Applied Genetics2024, 137(5): 113
30. HuaL*, SongR*, HaoX*, …DongY, WangX#, ZhangC#,ChenS#. Manipulation of the brown glume and internode 1 gene leads to alterations in lignified tissue coloration, lignification, and pathogen resistance in wheat. Plant Biotechnology Journal 2024,https://doi.org/10.1101/2024.10.11.617803
29. LiH*, Shams R*, SongR, QiaoL, …WangL#, ChenS#. Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa.Scientific Data 2024, https://doi.org/10.1101/2024.10.15.618371
28. LiH*; LiK*; LiHongna*; YangC; …AyliffeM; YuH#; ChenS#. Mapping and Candidate Gene Analysis of an All-Stage Stem Rust Resistance Gene in Durum Wheat Landrace PI 94701. PLANTS 2024,13(16): 2197
27. Shams R*; QiaoL; ShenT; HuaL; LiH; ZishanA; ChenS#. Exploring the Frontier of Wheat Rust Resistance: Latest Approaches, Mechanisms, and Novel Insights. PLANTS 2024,13(17):2502
26. Wang X*, Li H*, Shen T, … …, Wang X, Qu X, Chen S#, Guo L#. A near-complete genome sequence of einkorn wheat provides insight into the evolution of wheat A subgenomes. Plant Communications,2024, 2024,5(5):100768
25. Liu Y, Hou S, Chen S. Kinase fusion proteins: intracellular R-proteins in plant immunity. Trends in Plant Science, 2024, 29(3):278-282
24. Li H*, Hua L*, Zhao S*, Hao M, … …, WangX#, Chen S#. Cloning of the wheat leaf rust resistance gene Lr47introgressed from Aegilops speltoides. Nature Communications, 2023, 14(1): 6072
23. Zhang J*, Nirmala J*, Chen S*, JostM*,… …, Zhang P#, Rouse M#, LagudahE#. Single amino acid change can alter the specificity of the multi-allelic wheat stem rust resistance locus SR9. Nature Communications, 2023, 14: 7354
22. Wang J, Li H, Shen T, LyuS, … …, Rouse M#, Chen S#. High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155B1. The Crop Journal,202311(6), 1852-1861
21. Chang C, Yang S, Zhang M, … …, Chen S, Xie C, Ni Z, Sun Q, Gou J#. Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat. Plant Communications, 2023, 4(5): 100608
20. Li H, Luo J, Zhang W, Hua L, Li K, Wang J, Xu B, Yang C, Wang G, Rouse M, Dubcovsky J#, Chen S#. High-resolution mapping of SrTm4, a recessive resistance gene to wheat stem rust, Theoretical and Applied Genetics, 2023, 136(5): 120
19. Li C, Fan R, Ma C, Zhang Z, … …, Chen S, Zhou Y, Song C, Chen X#, Li H#. Reciprocal translocations hidden by phenotype and genotype within the same wheat cultivar. Crop Science, 2023, 63(5): 2727-2739
18. Huang L, Li Y, Chen S#, Periyannan S#, Fahima Z#, Editorial: Advances in crop resistance breeding using modern genomic tools. Frontiers in Plant Science, 2023, DOI:10.3389/fpls.2023.1143689
17. Ren X, Wang C, Ren Z, Wang J, Zhang P, Zhao S, Chen S#, Wang X#. Genetics ofResistance to Leaf Rust in Wheat: An Overview in a Genome-WideLevel.Sustainability, 2023,15(4), 3247
16. BaiS, Pang S, Li H, Yang J, Yu H, Chen S#, Wang X#. Broad-Spectrum Resistance to Leaf Rust in the Argentinean Wheat Cultivar “Klein Proteo” Is Controlled by LrKP Located on Chromosome 2BS. Agriculture, 2022, 12(11): 1836
15. Li M, Zhao S, Yang J, Ren Y, ... Chen S, Yu X, Chen F, Wang X. Exogenous expression of barley HvWRKY6 in wheat improves broad-spectrum resistance to leaf rust, Fusarium crown rot, and sharp eyespot. International Journal of Biological Macromolecules,2022, 218: 1002-1012
14. Jiang D, Hua L, Zhang C, Li H, Wang Z, Li J, ... Dubcovsky J, Chen S#, Mutations in the miRNA165/166 binding site of the HB2 gene result in pleiotropic effects on morphological traits in wheat. The Crop Journal, 2022,11(1): 9-20 (Cover story)
13. Wang J, Liu H, Zhao C, Tang H, …Chen S, Wei Y, Zheng Y, Lan X, Ma J. Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat. The Plant Genome, 2022, 15(4), p.e20252
12. Kuzay S, Lin H, Li C, Chen S, Woods DP, Zhang J, Dubcovsky J. WAPO-A1 is the causal gene of the 7AL QTL for spikelet number per spike in wheat. PLoS Genetics, 2022.18(1): e1009747
11. Luo J, Rouse MN, Hua L, Li H, Li B, Li T, ... Dubcovsky J,Chen S#. Identification and characterization of Sr22b, a new allele of the wheat stem rust resistance gene Sr22 effective against the Ug99 race group, Plant Biotechnology Journal, 2021, 20(3): 554-563
10. Li H, Hua L, Rouse MN, Li T, Pang S, Bai S, Shen T, Luo J, Li H, Zhang W, Wang X, Dubcovsky J, Chen S#, Mapping and characterization of a wheat stem rust resistance gene in durum wheat "Kronos". Frontiers in Plant Science, 2021, 12: 751398
9. Su J, Zhao J, Zhao S, Li M, Pang S, Kang Z, Zhen W, Chen S, Chen F, Wang X, Genetics of Resistance to Common Root Rot (Spot Blotch), Fusarium Crown Rot, and Sharp Eyespot in Wheat. Frontiers in Genetics, 2021, 12: p.699342
8.Chen S*#, Hegarty J, Shen T, Hua L, Li H, Luo J, Li H, Bai S, Zhang C, Dubcovsky J, Stripe rust resistance gene Yr34 (synonym Yr48) is located within a distal translocation of Triticum monococcum chromosome 5AmL into common wheat; Theoretical and Applied Genetics; 2021, 134: 2197-2211
7. Chen S*, Rouse M, Zhang W, Zhang X, Guo Y, Dubcovsky J, Wheat gene Sr60 encodes a protein with two putative kinase domains that confers resistance to stem rust,New Phytologist, 2020, 225: 948–959
6. Chen S*, Zhang W, Stephen B, Rouse M, Dubcovsky J, Identification and characterization of wheat stem rust resistance gene Sr21 effective against Ug99 race group at high temperature,PloS Genetics, 2018, 14(4): e1007287
5. Valentina K, Elitsur Y, Lin H, Dina R, Andrii F, Chen S, Feng L, Frenkel Z, ... Fahima T, Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family,Nature Communications, 2018, 9(1): 1-12
4. Chen S*, Yan G, Briggs J, Dubach F, Chao S, Zhang W, Rouse M, Dubcovsky J, Mapping and characterization of wheat stem rust resistance genes SrTm5 and Sr60 from Triticum monococcum,Theoretical and Applied Genetics, 2018, 131.3: 625-635
3. Zhang W*, Chen S*, Abate Z, Nirmala J, Rouse M, and Dubcovsky J, Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group,Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(45), E9483-E9492
2. Briggs J*, Chen S*, Zhang W, Nelson S, Dubcovsky J, Rouse M, Mapping of SrTm4, a Recessive Stem Rust Resistance Gene from Diploid Wheat Effective to Ug99,Phytopathology, 2015, 105.10: 1347-1354
1. Chen S*, Rouse M, Zhang W, Jin Y, Akhunov E, Wei Y, Dubcovsky J, Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group,Theoretical and Applied Genetics, 2015: 1-12
代表性专利：
8. 小麦叶锈病抗性蛋白、抗性基因及其应用；陈时盛等；2024107672380
7. 小麦叶锈病抗性蛋白 Lr.ace-4A、抗性基因与应用；陈时盛等；202411102330
6. 检测小麦连锁抗锈病基因Lr53和Yr35的分子标记、试剂盒、检测方法及其应用；陈时盛等；2024101384248
5.检测小麦Su1-Ph1基因的分子标记、部分同源染色体重组小麦的制备方法及应用；王桂平,李浩,李洪雨,陈时盛等；2023112839261
4.小麦叶锈病抗性蛋白及其编码基因和应用；陈时盛等；ZL 2022 1 1247709.2
3.小麦秆锈病抗性蛋白及其编码基因和应用；陈时盛等；ZL 2021 1 0969875.2
2.检测小麦抗叶锈病基因Lr47的分子标记、检测方法及其应用；陈时盛等；ZL 2022 1 0114498.9
1.一种检测小麦秆锈病抗性基因SrTm5的诊断性分子标记及其育种应用；陈时盛等；ZL202011374520.0
代表性科研项目：
1. PI，2025-2028，来源于山羊草属的连锁抗锈病基因Yr35和Lr53的克隆及其育种利用；国家自然科学基金面上项目；32472159；58万
2. PI，2024-2027，小麦远缘杂交新工具基因的研发和特色新品种培育；山东省重点研发计划（农业良种工程）；2024LZGC034；200万
3. PI，2023-2028，小麦近缘种优异抗病基因转移利用；山东省国家领军支持项目2023GJJLJRC-084；125万
4. PI，2022-2025，小麦近缘物种优异功能基因的发掘克隆与利用；国家高层次人才特殊支持计划；SQ2022QB01387；190万
5. CO-PI（子课题），2022-2027，基于大片段的小麦多基因高效聚合；国家重点研发青年科学家项目；2022YFD1201300；60万
6. PI，2022-2025，山东省泰山学者青年科学家项目；75万
7. PI，2022-2024，小麦广谱抗条锈病基因Yr34/Yr48的克隆及其抗病机制初探；山东省自然科学基金面上项目；ZR2021MC056；10万
8. PI，2021-2024，几个锈病抗性基因的发掘及其育种利用；鸢都产业领军人才项目；100万
植物新品种权与新种质：
1. 陈时盛等，小麦新品系“北大219”，植物新品种权20241000662；
2. 陈时盛等，小麦新品系“北大2260”，植物新品种权20241000660；
3. 陈时盛等，小麦新品系“北农2301”，植物新品种权20241000659；
4. 陈时盛等，小麦新品系“潍麦2222”，植物新品种权20241003899；
5. 小麦新种质：ZM032711 (Pku-Sr22b；中国种质资源库)；ZM032712 (Pku-SrKN；中国种质资源库)；PI 689563 (Sr60 introgression；美国种质资源库)；PI 700734 (Sr9e-h2 introgression；美国种质资源库); PI 700735 (Sr22b introgression；美国种质资源库)；Pku-Lr47(发放几十个科研或育种单位)。