Principal Investigator
Principal Investigator
Education
2009-2015,PhD in Biochemistry and Molecular Biology, Sichuan Agricultural University/University of California, Davis
2005-2009, BS in Biotechnology, Sichuan Agricultural University, China
Working Experience
April2019 – now, Principal Investigator, Peking University Institute of Advanced Agricultural Sciences, China
June2015 – March2019,Postdoctoral researcher, University of California, Davis, USA
Research Interests
l Collection and evaluation of wheat germplasm resource
l Identification and characterization of disease resistance in wheat and its wild relatives
l Wide hybridization, germplasm enhancement, and genetic improvement of wheat
Selected Publications
34. Shengsheng Bai#; Guiping Wang#; Rui Song; …Lihua Zhao; Yan Xue; Zheng Wang*; Shisheng Chen*. Mutations in wheat TaAPA2 gene result in pleiotropic effects on plant architecture. SCIENCE CHINA-LIFE SCIENCES2024,67:2039–2042
33. Xiangfeng Wang#; Hongna Li#; Tao Shen;…Xiaojian Qu; Shisheng Chen*; Li Guo*. A near-complete genome sequence of einkorn wheat provides insight into the evolution of wheat A subgenomes. PLANT COMMUNICATIONS2024,5(5):100768
32. Lei Hua#, Rui Song#, Xiaohua Hao#, …Yuxiu Dong, Xiaodong Wang, Chaozhong Zhang,Shisheng Chen*. Manipulation of the brown glume and internode 1 gene leads to alterations in lignified tissue coloration, lignification, and pathogen resistance in wheat. Plant Biotechnology Journal 2024,https://doi.org/10.1101/2024.10.11.617803
31. Hongna Li#, Shams ur Rehman#, Rui Song, Liang Qiao, …Le Wang,Shisheng Chen*. Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa.Scientific Data 2024, https://doi.org/10.1101/2024.10.15.618371
30. Binyang Xu#; Tao Shen#; Hong Chen#; Hongna Li; …Guo-Yue Chen; Ming Hao*; Shisheng Chen*. Mapping and characterization of rust resistance genes Lr53 and Yr35introgressed from Aegilops species. Theoretical and Applied Genetics2024, 137(5): 113
29. Hongyu Li#; Kairong Li#; Hongna Li; Chen Yang; …Michael Ayliffe; Haitao Yu*; Shisheng Chen*.Mapping and Candidate Gene Analysis of an All-Stage Stem Rust Resistance Gene in Durum Wheat Landrace PI 94701. PLANTS2024,13(16): 2197
28. Shams ur Rehman#; Liang Qiao;Tao Shen; Lei Hua; Hongna Li; Ahmad Zishan; Shisheng Chen*. Exploring the Frontier of Wheat Rust Resistance: Latest Approaches, Mechanisms, and Novel Insights. PLANTS2024,13(17):2502
27. Yukun Liu#; Shuguo Hou#; Shisheng Chen. Kinase fusion proteins: intracellular R-proteins in plant immunity. TRENDS IN PLANT SCIENCE2024, 29(3):278-282
26. Hongna Li#, Lei Hua#, Shuqing Zhao#, Ming Hao, … …, Xiaodong Wang*, Shisheng Chen*.Cloning of the wheat leaf rust resistance gene Lr47introgressed from Aegilops speltoides.Nature Communications 2023, 14(1): 6072
25. Jianping Zhang#, Jayaveeramuthu Nirmala#, Shisheng Chen#, Matthias Jost#,… …,Peng Zhang*, Matthew N. Rouse*, Evans Lagudah*. Single amino acid change can alter the specificity of the multi-allelic wheat stem rust resistance locus SR9. Nature Communications 2023, 14: 7354
24. Hongna Li#, Jing Luo#, Wenjun Zhang, LeiHua, … …,JorgeDubcovsky*, Shisheng Chen*. High-resolution mapping of SrTm4, a recessive resistance gene to wheat stem rust. Theoretical and Applied Genetics2023, 136(5): 120
23. Jian Wang#, Hongyu Li#, Tao Shen#,Shikai Lyu,… …, Matthew N. Rouse*, Shisheng Chen*. High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155B1. The Crop Journal202311(6), 1852-1861
22. Can Li, Ruixiao Fan, Chongyang Ma, … Shisheng Chen, Yun Zhou, Chun-Peng Song, Xiaojie Chen, Hao Li; Reciprocal translocations hidden by phenotype and genotype within the same wheat cultivar; Crop Science 2023, 63(5): 2727-2739
21. Chao-Yan Chang#, Shu-Xian Yang#, Mei-Qi Zhang, …Shisheng Chen, Chaojie Xie, Zhongfu Ni, Qixin Sun, Jin-Ying Gou*; Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat; Plant Communications 2023, 4(5): 100608
20. DengjiJiang#, LeiHua#, Chaozhong Zhang#, Hongna Li, … …, HaoLi, JorgeDubcovsky, Shisheng Chen*. Mutations in the miRNA165/166 binding site of the HB2 gene result in pleiotropic effects on morphological traits in wheat. The Crop Journal 2023, 11(1): 9-20
19. LinHuang#, YinghuiLi#, ShishengChen*, SambasivamPeriyannan*, TzionFahima*; Advances in crop resistance breeding using modern genomic tools; Frontiers in Plant Science2023, 14: 1143689
18. XiaopengRen#, ChuyuanWang#, ZhuangRen#, JingWang, … …,Shisheng Chen*, XiaodongWang*.Genetics of resistance to leaf rust in wheat: an overview in a genome-wide level. Sustainability2023,15(4): 3247
17. JingLuo#, Matthew N.Rouse#, Hua Lei, … …,YanpengWang*, Jorge Dubcovsky*, Shisheng Chen*. Identification and characterization of Sr22b, a new allele of the wheat stem rust resistance gene Sr22 effective against the Ug99 race group, Plant Biotechnology Journal2022, 20(3): 554–563
16. Shengsheng Bai#, Shuyong Pang#, Hongna Li, …Shisheng Chen*, Xiaodong Wang*; Broad-Spectrum Resistance to Leaf Rust in the Argentinean Wheat Cultivar “Klein Proteo” Is Controlled by LrKP Located on Chromosome 2BS; Agriculture2022, 12(11): 1836
15. Saarah Kuzay#, Huiqiong Lin#, Chengxia Li, Shisheng Chen, …Jorge Dubcovsky*; WAPO-A1 is the causal gene of the 7AL QTL for spikelet number per spike in wheat; PLoSGenetics 2022, 18(1): e1009747
14. Mengyu Li#, Shuqing Zhao#, Yang Junyu, …Shisheng Chen, Xiumei Yu, Feng Chen*, Xiaodong Wang*; Exogenous expression of barley HvWRKY6 in wheat improves broad-spectrum resistance to leaf rust, Fusarium crown rot, and sharp eyespot; International Journal of Biological Macromolecules 2022, 218: 1002-1012
13. Jian Wang#, Hang Liu#, Conghao Zhao, …Shisheng Chen, Yuming Wei, Youliang Zheng, Xiujin Lan*, Jian Ma*; Mapping and validation of major and stable QTL for flag leaf size from tetraploid wheat; The Plant Genome 2022, e20252
12. Hongna Li#, LeiHua#, Matthew N.Rouse, Tianya Li,… …, Jorge Dubcovsky*, Shisheng Chen*. Mapping and characterization of a wheat stem rust resistance gene in durum wheat “Kronos”. Frontiers in Plant Science2021, 12:751398
11.Shisheng Chen# *, Joshua Hegarty#, Tao Shen, Lei Hua, …, Chaozhong Zhang, Jorge Dubcovsky*. Stripe rust resistance gene Yr34 (synonym Yr48) is located within a distal translocation of Triticum monococcum chromosome 5AmL into common wheat.Theoretical and Applied Genetics 2021, 134: 2197–2211
10. Jun Su#, Jiaojie Zhao#, Shuqing Zhao#, …Shisheng Chen, Feng Chen*, Xiaodong Wang*; Genetics of Resistance to Common Root Rot (Spot Blotch), Fusarium Crown Rot, and Sharp Eyespot in Wheat. Frontiers in Genetics 2021, 12: 699342
9. Shisheng Chen#, Matthew N.Rouse, WenjunZhang, … …, Jordan Briggs, Jorge Dubcovsky*. Wheat gene Sr60 encodes a protein with two putative kinase domains that confers resistance to stem rust; New Phytologist2020, 225(2): 948-959
8. Shisheng Chen#, WenjunZhang, StephenBolus, Matthew N. Rouse, Jorge Dubcovsky*. Identification and characterization of wheat stem rust resistance gene Sr21 effective against Ug99 race group at high temperature.PloS Genetics2018, 14(4): e1007287
7. Valentina Klymiuk#, Elitsur Yaniv#, Lin Huang#,Dina Raats#, Andrii Fatiukha#,Shisheng Chen,…Schulman H. Alan, Tzion Fahima; Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family; Nature Communications 2018, 2041-1723
6. Shisheng Chen#, Yan Guo#, Jordan Briggs, Felix Dubach, … …, Matthew N. Rouse, Dubcovsky Jorge*. Mapping and characterization of wheat stem rust resistance genes SrTm5 and Sr60 from Triticum monococcum. Theoretical and Applied Genetics 2018, 131.3: 625-635
5. WenjunZhang#, Shisheng Chen#, Zewdie Abate, Jayaveeramuthu Nirmala, Matthew N. Rouse*, Jorge Dubcovsky*. Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group. PNAS 2017, 114(45), E9483-E9492
4. Jordan Briggs#, Shisheng Chen#, Wenjun Zhang, Sarah Nelson, Jorge Dubcovsky*, Rouse N. Matthew*; Mapping of SrTm4,a recessive stem rust resistance gene effective against races of the Ug99 group; Phytopathology 2015, 105.10: 1347-1354
3. Shisheng Chen#, Rouse N. Matthew#, Wenjun Zhang, Yue Jin, Eduard Akhunov, Yuming Wei, Jorge Dubcovsky*; Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group; Theoretical and Applied Genetics 2015: 1-12
2. Shisheng Chen#, Guo-Yue Chen#, Yang Cheng, Wu Wen-Xiong, …Liu Deng-Cai, Lan Xiu-Jin, Zheng You-Liang*; Identification and mapping of a stripe rust resistance gene in spring wheat germplasm HRMSN-81 from CIMMYT; Crop & Pasture Science 2013, 64,1-8
1.Shisheng Chen#, Guo-Yue Chen#, Hua Chen, …You-Liang Zheng*; Mapping a stripe rust resistance gene YrSphin wheat line D31 derived from Tritium sphaerococcum Perc.with SSR, SRAP and TRAP markers; Euphytica 2012, 185(1): 19-26
Patents
1. Shisheng Chen,Hongna Li,Lei Hua,Xiaodong Wang, Rui Song, Yanna Liu; Wheat leaf rust resistance protein, its coding gene and application;ZL 2022 1 1247709.2;8-18-2023;CN 116253784 B; China
2. Shisheng Chen,Hongna Li,Guiping Wang, Lei Hua, Tao Shen, Hongyu Li, Dengji Jiang, Jing Luo, Shengsheng Bai; A diagnostic molecular marker for Lr47, its detection method and application; ZL 2022 1 0114498.9; 11-9-2022; CN 114480709 B; China
3. Shisheng Chen,Hongna Li,Lei Hua,Shuyong Pang,Guiping Wang, Dengji Jiang, Keke Wei, Yanna Liu; Wheat stem rust resistance protein, its coding gene and application; ZL 2021 1 0969875.2; 5-2-2023; CN 114644703 B; China
4. Shisheng Chen, Jing Luo, Lei Hua; A diagnostic molecular marker for SrTm5, its detection method and application; ZL 2020 1 1374520.0; 7-12-2022; CN 112359130 B; China
5. Youliang Zheng, Guoyue Chen, Shisheng Chen,Yuming Wei; Specific molecular marker linked to the stripe rust resistance gene in wheat landrace “Yilongtuotuomai”and their applications; ZL 2012 10479255.1; China
6. Youliang Zheng, Guoyue Chen, Shisheng Chen, Yuming Wei; Molecular marker linked to the wheat stripe rust resistance gene, their acquisition methods, and applications; ZL201210407964.9; China
7. Shisheng Chen,Tao Shen, Lei Hua,Jing Zhang; The resistance proteins, resistance genes, and their applications in wheat leaf rust disease; 2024107672380; China
8. Shisheng Chen,Jinwei Yang, Hongna Li,Rui Song; Resistance protein Lr.ace-4A, resistance genes, and their applications in wheat leaf rust disease; 202411102330; China
9.Shisheng Chen,Hongna Li, Binyang Xu,Tao Shen; A diagnostic molecular marker for Lr53 and Yr35, its detection method and application; 2024101384248; China
10. Guiping Wang, Hao Li, Hongyu Li, Shisheng Chen; A molecular marker for detecting the wheat Su1-Ph1 gene, methods for inducing homoeologous recombination, and their applications; 2023112839261; China
WheatVariety PVPs and Germplasm
1. Shisheng Chenet al. Wheat variety ‘Beida219’. PVP 20241000662;
2. Shisheng Chen et al. Wheat variety‘Beida2260’.PVP 20241000660;
3. Shisheng Chen et al.Wheat variety‘Beinong2301’.PVP 20241000659;
4. Shisheng Chen et al.Wheat variety‘Weimai2222’.PVP20241003899.
5. Germplasm: ZM032711 (Pku-Sr22b); ZM032712 (Pku-SrKN); PI 689563 (Sr60 introgression line); PI 700734 (Sr9e-h2 introgression line); PI 700735 (Sr22b introgression line).
Research Projects
1. PI, 2025-2028, Cloning of the linked rust resistance genes Yr35 and Lr53 introgressed from Aegilops species and their deployment in wheat breeding; National Natural Science Foundation of China;32472159; 0.58 million¥.
2. PI, 2024-2027, Development of novel genetic tools for distant hybridization in wheat and the cultivation of new varieties; Key R&D Program of Shandong Province; 2024LZGC034; 2 million¥.
3. PI, 2023-2028, Transfer and utilization of functionalgenes from closely related wheat species; National Leading Support Project of Shandong Province; 2023GJJLJRC-084; 1.25 million¥.
4. PI, 2022-2025, Cloning of diseaseresistance genes from wheat wild relatives; National youth talent support program;SQ2022QB01387; 1.9 million¥.
5. CO-PI, 2022-2027, Transgene cassettes confer broad-spectrum resistance to pathogens in wheat, National Key Research andDevelopment Program of China; 2022YFD1201300; 2 million¥.
6. PI, 2022-2025, Young Taishan Scholars Program of Shandong Province, 0.75million¥.
7. PI, 2021-2024, Yuanduindustry leading talents, 1 million¥.
8. PI, 2022-2024, Cloning of thestripe rust resistance gene Yr34 introgressed from T. monococcum;Provincial Natural Science Foundation of Shandong; ZR2021MC056; 100 thousand ¥.
Honorables and Awards
1. The national youth talent support program, 2022
2. The Young Taishan Scholars Program of Shandong Province, 2022
3. The Model Worker of ShandongProvince, 2023
4. The special government allowance, 2024
Conferences and Invited Talks
1. Shisheng Chen, 2024, Harnessing the diversity of wild wheat for disease resistance; The 8th Yellow River International Forum; Zhengzhou, Henan, China.
2. Shisheng Chen, 2024,Discovery, cloning, and utilization of superior genes derived from Aegilops species;The 2nd Summit Forum on Wheat Bio-Breeding; Jinan, Shandong, China.
3. Shisheng Chen, 2023,Identification and characterization of rust resistance genes from wild relatives of wheat; The 8th Wheat Genetics and Breeding Symposium and the 11th National Conference on Wheat Genomics and Molecular Breeding; Hefei, Anhui, China.
4. Shisheng Chen, 2023,Cloning of the wheat leaf rust resistance gene Lr47introgressed from Aegilops speltoides; The 3rd Wheat Omics Youth Academic Symposium and National Young Wheat Breeders Seminar; Qingdao, Shandong, China.
5. Shisheng Chen, 2022,Cloning rust resistance from bread wheat progenitors; The International Academic Conference on Triticeae Crops Production and Challenges; Online.
6. Shisheng Chen, 2022, Challenges and solutions for wheat diseases in China; The 1st Peking University Forum on Modern Agriculture; Online.
7. Shisheng Chen, 2021,Challenges and opportunities in wheat R gene cloning; The 6th International Conference on Biotic Plant Interactions; Online.
8. Shisheng Chen, 2021,Discovery and utilization ofrust resistance genes;The "Agriculture Flourishes by Huai River" Technology Development Forum; Huaian, Jiangsu, China.
9. Shisheng Chen, 2021,Research on stem rust and stripe rust resistance genes from Triticum monococcum; The 2nd Wheat Omics Youth Academic Symposium; Yangling, Shanxi, China.
10. Shisheng Chen, 2021, Research advances in cloning of wheat stem rust resistance genes; The "Northwest A&F University-Corteva" Crop Green Production Forum; Online.
11. Shisheng Chen, 2021,Enhancing disease resistance in hybrid wheat; The 710th Academic Symposium of the Xiangshan Science Conferences; Beijing, China.
12. Shisheng Chen, 2019,Map-based cloning of wheat stem rust resistance genes effective against the Ug99 race group; International Forum on Modern Agriculture and Rural Revitalization;Taian, Shandong, China.