Platform name

Cryo-Electron Microscopy Facility

 

Platform leader

Dr. Jun Zhao

 

Key Facilities

 

This facility provides critical electron microscopy facility service for Peking University Institute of Advanced Agricultural Sciences. Additionally, the Cryo-Electron Microscopy Facility provides the technical support for single particle, electron tomography, and cell/tissue biology research.

 

At present, the platform is equipped with three electron microscopes, including Talos 120C for negative staining, one medium Glacios (200kV) and one Titan Krios (300kV) with a K3 direct electron detector and a BioQuantum energy filter. The facility also provides related sample preparation instruments including: light microscope fluorescent CorrSight; high pressure freezing; plunge freezing Vitrobot; freeze-substitution AFS; cryo-ultramicrotomy; and a plasma cleaning system. In addition, the platform has a set of high-performance computer clusters for image data storage, processing and reconstruction, offering services to cover the entire Cryo-EM workflow.

  

 

Services Provided

 

	Consortium   rates	External   Academic rates*
300 kV   Titan Krios	$900   per day	$1800   per day
200 kVGlacios	$400   per day ($40 per hour)	$800   per day ($80 per hour)
Cryo-FIB   Helios	$400   per day ($40 per hour)	$800   per day ($80 per hour)
Consumables such   as clipping components, autogrid boxes, and hard drives will be charged at   cost.

 

 

Cases

 

Yuxuan Zhang#, Xiaoli Lin#, Chengying Ma#, Jun Zhao#, Xiaojin Shang, Zhengdong Wang, Bin Xu, Ning Gao, Xing Wang Deng*, Jizong Wang*. 2023. Structural insights into plant phytochrome A as a highly sensitized photoreceptor. Cell Research 33:806-809.

 

Yuanhao Cheng, Xiaojun Huang, Bin Xu, Wei Ding*. 2023. AutoEMage: automatic data transfer, preprocessing, real-time display and monitoring in cryo-EM. Journal of Applied Crystallography. 56. 10.1107/S1600576723008257.

 

After each data collection, staff will provide a summary of optics and software parameters used on each specific instrument. Please acknowledge the support of the IAAS Cryo-EM facility in research publications arising from use of the center. This acknowledgment should include the following or similar: “Cryo-EM data were collected at the Cryo-EM facility of Peking University Institute of Advanced Agricultural Sciences”.

 

Contact

Platform Leader: Dr. Jun Zhao

E-mail: jun.zhao@pku-iaas.edu.cn